Cytometry and Cell Sorting Core

Overview of Services


The Cytometry and Cell Sorting Core (CCSC) facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting.


This site provides information about specific equipment, services, fees, training, methods, and resources.


What Is Flow Cytometry?

Flow cytometry uses fluorescent probes to identify and characterize cells or particles. Cells or particles tagged with fluorescent molecules enter the cytometer via a fluid stream. The cells then pass by a laser, which emits a specific wavelength of light. The fluorescent probes are excited by the laser and then emit light. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is sent to the computer. Information about the size and granularity of a cell is recorded, as well. The result is a visual presentation describing an individual or group of cellular events. The cells or particles can be separated by sorting, or the information can be collected and analyzed.


What is Cell Sorting?

Cell sorting is the separation and isolation of various cell populations. There are two methods for performing cell sorting: One is by using flow cytometry and the other is by magnetically labeling the cells to differentiate and separate the cell populations. Using flow cytometry requires a Flow Cytometric Cell Sorter like the BD FACSAria. Sorting on a cytometer is similar to standard flow cytometry except that after fluorescent analysis the stream is vibrated at a specific frequency to separate it into droplets. These droplets are then charged or not depending upon the fluorescent profile of the cell within. The drops go through an electric field that sends the charged drops of interest into a tube or plate leaving unwanted cells to go to the waste. In contrast the magnetic separation uses a column that is placed under a magnetic field to retain cells labeled with magnetic beads. Cells are loaded onto the column and the labeled cells are retained in the magnetic field while the unlabeled cells pass through. The column can then be removed from the magnetic field to remove the labeled cells, and either the negative or positive fractions can be processed for experimental purposes.



Getting Started


  • BCM Users:  Click the link in the upper right corner to login or register for an iLab account using valid BCM credentials.

  • Non-BCM Users:  Click the appropriate link in the upper right corner to login with your approved iLab credentials or to sign up for an iLab account.






Joel M. Sederstrom, M.S. -  Core Director

Office: T103A

Phone: 713-798-3868



Lab Phone - T103:  713-798-3868

Lab Fax - T103:  713-798-9009


Location and Hours of Operation





Taub 103

One Baylor Plaza

Houston, TX 77030


Assisted Services:  9 a.m. - 6 p.m., M - F

Unassisted/Self-Use: 24/7

Links and Resources




Name Role Phone Email Location
Joel M. Sederstrom
T103, T105
Amanda White
Cytometry Technologist II
T103, T105
Brandon Saxton
Cytometry Technologist I
T103, T105

Available Equipment and Resources

Name Price
Tech Availabilities: Assisted Use, Training, Consultation (VIEW ONLY) View calendar
Cell Sorters: AriaI(M901), AriaII(T105), CAGT AriaIII(M903) View calendar
Cell Sorter: Sony SH800(T103) View calendar
Cell Analyzers: LSRII(T103), LSRFortessa(T103), LSRII(M902), optional HTS View calendar
Cell Analyzers: BD CantoII Yellow-Green Carousel(T103), BD CantoII Violet-HTS(T103), and AttuneNXT(T103) View calendar
Cell Imager: ImageStreamX MarkII(T103) View calendar
ViCell(T103) View calendar
Cell Separator: AutoMACS Pro (M902) View calendar
Data Analysis Workstation (PC T103 and PC M902) View calendar
CCSC Analyzer Training Course (view only - core will schedule) View calendar
TCH Cell Sorter (BioRad S3) View calendar
TCH Cell Sorter (BD Aria Fusion) View calendar
TCH Cell Analyzers: BD LSRII, Guava easyCyte. View calendar
CALENDAR NOT IN USE- Cell Imager and Separator: DEP Array(T105) View calendar


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