Cytometry and Cell Sorting Core

Overview of Services

 

The Cytometry and Cell Sorting Core (CCSC) facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting.

 

This site provides information about specific equipment, services, fees, training, methods, and resources.

 

What Is Flow Cytometry?

Flow cytometry uses fluorescent probes to identify and characterize cells or particles. Cells or particles tagged with fluorescent molecules enter the cytometer via a fluid stream. The cells then pass by a laser, which emits a specific wavelength of light. The fluorescent probes are excited by the laser and then emit light. The fluorescent signal is detected and amplified, then translated into an electronic signal, which is sent to the computer. Information about the size and granularity of a cell is recorded, as well. The result is a visual presentation describing an individual or group of cellular events. The cells or particles can be separated by sorting, or the information can be collected and analyzed.

 

What is Cell Sorting?

Cell sorting is the separation and isolation of various cell populations. There are two methods for performing cell sorting: One is by using flow cytometry and the other is by magnetically labeling the cells to differentiate and separate the cell populations. Using flow cytometry requires a Flow Cytometric Cell Sorter like the BD FACSAria. Sorting on a cytometer is similar to standard flow cytometry except that after fluorescent analysis the stream is vibrated at a specific frequency to separate it into droplets. These droplets are then charged or not depending upon the fluorescent profile of the cell within. The drops go through an electric field that sends the charged drops of interest into a tube or plate leaving unwanted cells to go to the waste. In contrast the magnetic separation uses a column that is placed under a magnetic field to retain cells labeled with magnetic beads. Cells are loaded onto the column and the labeled cells are retained in the magnetic field while the unlabeled cells pass through. The column can then be removed from the magnetic field to remove the labeled cells, and either the negative or positive fractions can be processed for experimental purposes.

 

 

Getting Started

 

  • BCM Users:  Click the link in the upper right corner to login or register for an iLab account using valid BCM credentials.


  • Non-BCM Users:  Click the appropriate link in the upper right corner to login with your approved iLab credentials or to sign up for an iLab account.

 

 

 

Leadership

 

Joel M. Sederstrom, M.S. -  Core Director

Office: T103A

Phone: 713-798-3774

Email: sederstr@bcm.edu

 

Lab Phone - T103:  713-798-3868

Lab Fax - T103:  713-798-9009

 


Location and Hours of Operation

 

Location

 

Hours

Taub 103

One Baylor Plaza

Houston, TX 77030

 

Assisted Services:  9 a.m. - 6 p.m., M - F

Unassisted/Self-Use: 24/7

Links and Resources

 

    

Contacts

Name Role Phone Email Location
Joel M. Sederstrom
Director
 
713-798-3774
 
sederstr@bcm.edu
 
T103, T105
 
Amanda White
Cytometry Technologist II
 
713-798-3868
 
anwhite@bcm.edu
 
T103, T105
 
Brandon Saxton
Cytometry Technologist I
 
713-798-3868
 
saxton@bcm.edu
 
T103, T105
 
Erin Leishman
Cytometry Technologist I
 
713-798-3868
 
eecox@bcm.edu
 
T103
 

Schedulable Equipment and Resources

Tech Availabilities: Assisted Use, Training, Consultation View calendar
Cell Sorters: AriaI(M901), AriaII(T105), CAGT AriaIII(M903) View calendar
Cell Analyzers: LSRII(T103), LSRFortessa(T103), LSRII(M902), optional HTS View calendar
Cell Analyzers: BD CantoII(T103) and Attune(T103) View calendar
ViCell(T103) View calendar
Cell Separator: AutoMacs(T105) View calendar
Cell Separator: DEP Array(T613) View calendar
Data Analysis Workstations(T103) View calendar
TCH MoFlo Cell Sorter View calendar
TCH Cell Analyzers View calendar
CCSC Analyzer Training Course (view only - core will schedule) View calendar
Cell Sorter: CAGT AriaIII(M903) View calendar
Cell Analyzer: Attune Acoustic Focusing Cytometer(T103) View calendar